منابع مشابه
Hepatitis C Virus RNA Functionally Sequesters miR-122
Hepatitis C virus (HCV) uniquely requires the liver-specific microRNA-122 for replication, yet global effects on endogenous miRNA targets during infection are unexplored. Here, high-throughput sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO binding on the HCV 5'UTR at known and predicted miR-122 sites. On th...
متن کاملHepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors
Hepatitis C virus (HCV) replication is dependent on a liver-specific microRNA (miRNA), miR-122. A recent clinical trial reported that transient inhibition of miR-122 reduced viral titres in HCV-infected patients. Here we set out to better understand how miR-122 inhibition influences HCV replication over time. Unexpectedly, we observed the emergence of an HCV variant that is resistant to miR-122...
متن کاملmiR-122 is more than a shield for the hepatitis C virus genome.
S ince the discovery in 2005 of the essential role of the liver-specific microRNA, miR-122, in HCV replication (1), the mechanism by which it stimulates this process has proved elusive. In PNAS, Li et al. demonstrate thatmiR-122 acts to shield the HCV genome against degradation by the cytosolic RNA exonuclease, Xrn1 (2). Although this may be one way in which this microRNA promotes HCV replicati...
متن کاملCompetitive Binding between miR-122 and p68 onto Hepatitis C Viral RNA
BACKGROUND Liver-specific microRNA (miR)-122 has been shown to be involved in regulating translation of hepatitis C viral (HCV) RNA. This study aimed to explore the molecular mechanism of miR-122 in regulating HCV RNA translation initiation. MATERIAL/METHODS In human liver hepatocellular carcinoma cell line HepG2, UV cross-link assay was performed on a large scale to identify RNA-binding prot...
متن کاملViral Determinants of miR-122-Independent Hepatitis C Virus Replication
Hepatitis C virus (HCV) replication requires binding of the liver-specific microRNA (miRNA) miR-122 to two sites in the HCV 5' untranslated region (UTR). Although we and others have shown that viral genetics impact the amount of active miR-122 required for replication, it is unclear if HCV can replicate in the complete absence of this miRNA. To probe the absolute requirements for miR-122 and th...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2013
ISSN: 1547-6286,1555-8584
DOI: 10.4161/rna.25137